Ccoaomt
Mostrando 1-12 de 16 artigos, teses e dissertações.
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1. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum
ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great diffic
An. Acad. Bras. Ciênc.. Publicado em: 2018-03
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2. Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw
Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key en
Genet. Mol. Biol.. Publicado em: 25/10/2013
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3. Avaliação da expressão gênica diferencial entre folhas e tecidos vasculares de Eucalyptus grandis
No presente trabalho está descrita a análise de experimentos de microarranjos de DNA realizados pelos pesquisadores do Projeto GENOLYPTUS em conjunto com a empresa NimbleGen Systems Inc. (Reykjavik, Iceland), assim como a validação dos resultados gerados por essa análise, com a finalidade de encontrar genes diferencialmente expressos entre folhas e xile
Publicado em: 2008
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4. Proteoma comparativo de xilema de eucalyptus grandis e eucalyptus globulus
A quantidade e a qualidade da lignina, relacionada ao padrÃo dos molignÃis G/S, sÃo as maiores barreiras à produÃÃo de polpa de celulose. O papel dos genes envolvidos na biossÃntese de monolignÃis, como 4cl, cad, ccoaomt, comt, cald5h e pal tÃm sido descritos na literatura. Estudos proteomicps podem ser utilizados para o estudo da gÃnese da madeira
Publicado em: 2007
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5. Genes de lignificaÃÃo em eucalyptus: estrutura e diversidade genÃtica dos genes 4cl e ccoaomt
O presente trabalho teve como objetivo o estudo da diversidade nucleotÃdica em dois genes chave na via de biossÃntese de lignina. Os genes 4cl e ccoaomt foram estudados em amostras de populaÃÃes naturais de trÃs espÃcies comerciais de eucalipto, E. grandis, E. globulus e E. urophylla. A mineraÃÃo de um banco de dados de ESTs gerado no projeto Genolyp
Publicado em: 2005
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6. Sugarcane expressed sequences tags (ESTs) encoding enzymes involved in lignin biosynthesis pathways
Ligninas são compostos fenólicos encontrados nas paredes secundárias do sistema vascular vegetal e desempenham importantes papéis biológicos, reduzindo a permeabilidade da parede celular em relação à água, estando também envolvidas em mecanismos de defesa contra patógenos. A via metabólica da lignina e as enzimas envolvidas na sua síntese vem se
Genetics and Molecular Biology. Publicado em: 2001-12
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7. An alternative methylation pathway in lignin biosynthesis in Zinnia.
S-Adenosyl-L-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) is implicated in disease resistant response, but whether it is involved in lignin biosynthesis is not known. We isolated a cDNA clone for CCoAOMT in differentiating tracheary elements (TEs) induced from Zinnia-isolated mesophyll cells. RNA gel blot analysis showed that the expr
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8. Dual methylation pathways in lignin biosynthesis
Caffeoyl-coenzyme A (CoA) O-methyltransferase (CCoAOMT) has been proposed to be involved in an alternative methylation pathway of lignin biosynthesis. However, no direct evidence has been available to confirm that CCoAOMT is essential for lignin biosynthesis. To understand further the methylation steps in lignin biosynthesis, we used an antisense approach to
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9. Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants.
Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) was previously shown to be associated with lignification in both in vitro tracheary elements (TEs) and organs of zinnia (Zinnia elegans). However, it is not known whether this is a general pattern in dicot plants. To address this question, polyclonal antibodies against zinnia recombinant CCoAOMT fusion prot
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10. Essential Role of Caffeoyl Coenzyme A O-Methyltransferase in Lignin Biosynthesis in Woody Poplar Plants
Caffeoyl coenzyme A O-methyltransferase (CCoAOMT) has recently been shown to participate in lignin biosynthesis in herbacious tobacco plants. Here, we demonstrate that CCoAOMT is essential in lignin biosynthesis in woody poplar (Populus tremula × Populus alba) plants. In poplar stems, CCoAOMT was found to be expressed in all lignifying cells including vesse
American Society of Plant Physiologists.
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11. Cell-Specific and Conditional Expression of Caffeoyl-Coenzyme A-3-O-Methyltransferase in Poplar1
Caffeoyl coenzyme A-3-O-methyltransferase (CCoAOMT) plays an important role in lignin biosynthesis and is encoded by two genes in poplar (Populus trichocarpa). Here, we describe the expression pattern conferred by the two CCoAOMT promoters when fused to the gus-coding sequence in transgenic poplar (Populus tremula × Populus alba). Both genes were expressed
American Society of Plant Physiologists.
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12. Characterization and expression of caffeoyl-coenzyme A 3-O-methyltransferase proposed for the induced resistance response of Vitis vinifera L.
Cell-suspension cultures of Vitis vinifera L. cv Pinot Noir accumulated resveratrol upon fungal elicitation, and the activity of S-adenosyl-L-methionine:trans-caffeoyl-coenzyme A 3-O-methyl-transferase (CCoAOMT), yielding feruloyl-CoA, increased to a transient maximum at 12 to 15 h. CCoAOMT cDNA was cloned from the elicited cells and was shown to encode a po