Vigilância de epizootias em primatas não humanos (PNH) como instrumento de monitoramento de arboviroses e outras viroses de interesse em saúde pública

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Epizootics in wild animals can be considered important public health indicators, besides little used in epidemiological surveillance. Evaluation of non human primates (NHP), like ?sentinels animals?, was realized through this study in Porto Rico County region, upper Paraná River, including Paraná and Mato Grosso do Sul States, Brazil, between June/04 and April/06. Capture techniques were used for three native species: Alouatta caraya, Cebus nigritus and Cebus cay. A horse farm herd was used in present study because lived near by one of the PNH capture locals. Biological material (blood, sera, feces, and others) was collected, separated and used to evaluate sanitary status. Operational proceedings were standardized to do quick and ordinate epizootics surveillance. Four types of field work were proposed: two in passive way and two in active form. Saint Louis encephalitis virus (SLEV) (n=133 PNH; n=23 farm horses) and Borna disease virus (BDV) (n=147 PNH) were diagnosed. The antibodies prevalence against SLEV was 11.62% (A. caraya), 12.50% (C. nigritus), 30.77% (C. cay) by inhibition hemagglutination test. From these, 2.32% (A. caraya), 6.25% (C. nigritus) and 15.38% (C. cay) were confirmed by serum neutralization in mice. For farm horses the antibodies prevalence against SLEV was 39.13% by inhibition hemagglutination test, do not being confirmed by serum neutralization in mice. No arbovirus was isolated of 133 PNH blood samples and 23 farm horses blood samples. The BDV p24 RNA presence was investigated by reverse transcription and nested PCR using specific primers to p24 from BDV. Prevalence of p24 RNA of BDV in these monkeys was 15.38%. The obtained sequences revealed identity with sequential database deposited in GenBank to BDV. Only Cebus cay monkeys were positive to BDV. This result helps understanding the distribution of BDV and SLEV between monkey species and the possible role of these animals in virus environmental maintenance.

ASSUNTO(S)

vigilância epidemiológica encefalite de st. louis st. louis encephalitis primatas - doenças epidemiological surveillance

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