VALIDAÇÃO DE MÉTODO POR MEFS-HS/CG-EM PARA DETERMINAÇÃO DE CARBARIL EM PLASMA SANGUÍNEO

AUTOR(ES)

Souza, Francisco Thiago C. de, Camelo, André Luiz M., Marques, Ricardo de Araújo, F. Filho, Luiz Ivando Pires, Pinheiro, Ronald F., Longhinotti, Elisane, Becker, Helena

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2017-06

RESUMO

The aim this research is to develop a HS-SPME/GC-MS method to analyze the carbaryl in blood plasma samples of the rural workers, who live round the perimeter of Chapada do Apodi-CE. This was achieved by chromatographic signal of the 1-naftol, degradation product of carbaryl with the intention of evaluating the degree of exposure of the workers to pesticides, causing chromosome abnormalities as already evidence in appropriate literature. Three different types of covered SPME fibers were tested and a full factorial design with central point in two levels. The variables optimized were temperature and ionic strength and temperature was the response in the design. Study was undertaken to determinate the equilibrium time. The thermal degradation of carbaryl in SPME headspace was analyzed by 1H-NMR. Proposed method achieved low limits of detection and quantification (1.5 and 5 ng mL-1, respectively) with good precision and accuracy, using the method of weighted least squares. However, the proposed method can be considered a good alternative way of monitoring carbaryl in blood plasma by analytical signal of 1-naftol. None of the plasma analyzed presented a concentration of 1-naftol compound higher than limit of detection of the method proposed here.

Documentos Relacionados

• Desenvolvimento de um método de MEFS com CG-DIC para determinação de 1,4 dioxana em amostras de cosméticos
• Validação de método analítico por ELL-CG-EM para detecção de trialometanos decorrentes da cloração de águas contendo Microcystis
• Validação intralaboratorial de método para determinação de aflatoxina M1 em leite por cromatografia em camada delgada
• Otimização e validação intralaboratorial de método para a determinação de patulina em uva por Cromatografia em Camada Delgada
• Validação de método por cromatografia líquida de alta eficiência para determinação da lamivudina e zidovudina em comprimidos