Toll-like receptors expression in peripheral blood and oral mucosa of recurrent aphthous stomatitis / Expressão de receptores Toll-Like (2, 4 e 7) em células do sangue e da mucosa oral de pacientes portadores de ulceração aftosa recorrente

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Recurrent aphthous stomatitis (RAS) is one of the most common oral mucosal diseases which may cause serious impairment on patients quality of life. Despite its still unknown pathogenesis, several factors have been consistently associated with RAS, especially genetic and immunological changes, which has driven a lot of research effort towards these issues. Toll-Like receptors (TLR) recognize molecular products, mainly derived from microorganisms, triggering the inflammatory response. However, abnormalities in their function may give rise to the development of diseases, through abnormal activation of the immune system. The purpose of this investigation was to evaluate the expression of receptors TLR-2, TLR-4 and TLR-7 in peripheral blood and tissue samples of RAS and healthy controls, through real time RT-PCR technique. Five UAR patients and four healthy volunteers with no RAS history composed the casuistic and were submitted to biopsy of their UAR lesions or normal oral mucosa. On that same session patients had a blood sample taken through venopuncture. Both samples (tissue and blood) were, afterwards, submitted to lab procedures of RNA extraction and toll-like receptors expression through real time RT-PCR. There were no significant differences in the expression of these receptors between RAS and healthy controls in the two sample types. Further studies are necessary to allow sound conclusions on the actual participation of these receptors in RAS, since the sample followed were small and there are no similar studies published.

ASSUNTO(S)

toll-like receptors ulceração aftosa recorrente recurrent aphthous stomatitis rt-pcr em tempo real receptores toll-like real time rt-pcr

ACESSO AO ARTIGO

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