The use of lysozyme to prepare biologically active chitooligomers

AUTOR(ES)

Zimoch-Korzycka, Anna, Gardrat, Christian, Castellan, Alain, Coma, Véronique, Jarmoluk, Andrzej

FONTE

Polímeros

DATA DE PUBLICAÇÃO

2015-02

RESUMO

Two types of crustacean commercial chitosans (CS1, CS2) were dissolved in lactic acid solutions, hydrolysed by lysozyme and finally fractioned by methanol solutions into two parts containing chito-oligomers (CS-O1, CS-O2). The antioxidant power and antimicrobial properties of both fractions were studied and compared with non-hydrolysed CS1 and CS2. The antioxidant properties were determined by the ferric ion reducing antioxidant power (FRAP) method while the bioactive properties were evaluated against a strain of Listeria monocytogenes. CS-O obtained from the solid fraction of the chito-oligomers solid fractions treated with 90% methanol showed the highest reducing power. Microbiological tests showed that CS-O exhibit higher antilisterial activity than CS.

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