Use of Sublimation To Prepare Solid Microbial Media with Water-Insoluble Substrates
AUTOR(ES)
Alley, Jeremy F.
FONTE
American Society for Microbiology
RESUMO
A method was developed to deposit a visible layer of water-insoluble compounds via sublimation onto the surface of solid media. The compound is sublimed from a heated aluminum dish containing the compound onto the surface of an inverted, ice-cooled, inoculated agar petri dish. The method results in the deposition of a thin, even layer on the agar surface without the use of solvent. After incubation, clearing zones around colonies indicate the presence of compound-degrading microorganisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91845Documentos Relacionados
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