The use of internal dose biomarkers for assessment of lead exposure and its correlations with anemia and genetic polymorphisms from children living in uncontaminated area / Utilização de biomarcadores de dose interna para avaliação da exposição ao chumbo e suas correlações com anemia e polimorfismos geneticos em crianças residentes em uma região supostamente não contaminada
AUTOR(ES)
Glauce Regina Costa de Almeida
DATA DE PUBLICAÇÃO
2009
RESUMO
With increasing evidence of adverse health effects of lower levels of lead (below 10 $g/dL in whole blood), studies on novel internal dose biomarkers are needed. In Brazil, some studies showed high amounts of lead in the superficial enamel of deciduous enamel. The aim of this study was 1) to determine and to correlate the lead concentration in children living in Campos Elíseos district (in Ribeirao Preto, SP, Brazil), in whole blood (BL), serum, saliva, and surface enamel; 2) to correlate lead concentrations found using these biomarkers with anemia; 3) to evaluate the influence of ALAD and VDR polymorphisms on lead concentrations in these biomarkers. 444 children aged 6-8 years were enrolled in this study. BL, serum, parotid, submandibular and whole saliva were collected in trace element free tubes. Two successive acid etch enamel microbiopsies were performed on one deciduous and one permanent teeth. Phosphorus was colorimetrically determined to calculated microbiopsy depth. Lead concentrations were determined by inductively coupled plasma mass spectrometry (ICPMS). The detection limit (DL) was 0.03 $g/L (serum) and 0.02 $g/L (saliva). The hemoglobin level was determined by an automated cell counter (Micros - ABX). Genotypes for the ALAD and VDR polymorphism were determined by PCR and restriction fragment length digestion. For correlations, children were subdivided in two groups according to BL concentration (<4 and = 4 $g/dL) and enamel lead concentration (<400 and = 400 $g/g). Statistical significance was accepted when p<0.05. BL and serum lead levels ranged from 0.2 to 9.4 (median, 2.1) $g/dL and
- Relevancia de polimorfismos geneticos para concentrações sanguineas e plasmaticas de chumbo na gravidez
- Exposição a pesticidas organoclorados e desenvolvimento cognitivo em crianças e adolescentes residentes em uma área contaminada no Brasil
- Exposição a chumbo e comportamento anti-social em adolescentes
- Gender and Race/Ethnicity Differences in Lead Dose Biomarkers
- Influence of calcium supplementation on blood lead levels and markers of lead exposure in posmenopausal women
ASSUNTO(S)
serum anemia saliva anemia soro deciduous teeth saliva dentes deciduos
