The Drosophila Sex Determination Gene Snf Is Utilized for the Establishment of the Female-Specific Splicing Pattern of Sex-Lethal
AUTOR(ES)
Albrecht, E. B.
RESUMO
The Drosophila snf gene is a positive regulator of the sex determination gene Sex-lethal in both the germline and the soma. Its role in the soma is only evident when the probability of Sex-lethal activation has been reduced. For instance, in an otherwise wild-type background, females homozygous for a weak snf mutation produce both male and female progeny; however, when mated to males hemizygous for a null allele of Sex-lethal, they produce only male progeny. We demonstrate that the lack of female progeny is due to aberrant Sex-lethal regulation in late embryogenesis. In these mutant embryos, there is little accumulation of the late female-specific spliced RNAs and proteins. In contrast, in early embryogenesis, Sex-lethal regulation is not affected. The accumulation of both the early Sex-lethal transcripts and proteins is normal. These results suggest that the wild-type product of snf plays an important role in establishing the female-specific RNA splicing pattern of Sex-lethal. Whether snf influences the female-specific splice site choice directly or indirectly remains to be determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1205517Documentos Relacionados
- The Drosophila melanogaster fl(2)d gene is needed for the female-specific splicing of Sex-lethal RNA.
- A Female-Specific Lethal Lesion in an X-Linked Positive Regulator of the Drosophila Sex Determination Gene, SEX-LETHAL
- Control of Drosophila Sex-lethal pre-mRNA splicing by its own female-specific product.
- Multiple response elements in the Sex-lethal early promoter ensure its female-specific expression pattern.
- The Genetic Analysis of Snf: A Drosophila Sex Determination Gene Required for Activation of Sex-Lethal in Both the Germline and the Soma