The Drosophila melanogaster fl(2)d gene is needed for the female-specific splicing of Sex-lethal RNA.
AUTOR(ES)
Granadino, B
RESUMO
In Drosophila melanogaster, sex determination and dosage compensation are under the control of the Sex-lethal (Sxl) gene. We have identified a gene, female-lethal-2-d (fl(2)d), located in the second chromosome, that interacts with Sxl. fl(2)d homozygous clones, induced during the larval stage of fl(2)d/+ females, develop male structures instead of female ones. fl(2)d homozygous females hypertranscribe their two X chromosomes, as measured by comparing the level of the X-linked sgs-4 transcript, which is dosage compensated, with that of the autosomal sgs-3 transcript. Thus, with respect to the processes of sex determination and dosage compensation, loss-of-function mutations at the fl(2)d and at the Sxl genes are equivalent. Moreover, fl(2)d homozygous female larvae express the Sxl transcripts characteristic of males. These results indicate that the fl(2)d gene is needed for the sex-specific splicing pattern of the Sxl RNA that occurs in females, thus suggesting the involvement of the fl(2)d gene in the positive autoregulatory pathway of Sxl.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=552292Documentos Relacionados
- The Drosophila Sex Determination Gene Snf Is Utilized for the Establishment of the Female-Specific Splicing Pattern of Sex-Lethal
- Control of Drosophila Sex-lethal pre-mRNA splicing by its own female-specific product.
- A Female-Specific Lethal Lesion in an X-Linked Positive Regulator of the Drosophila Sex Determination Gene, SEX-LETHAL
- Evidence of a Dual Function in Fl(2)d, a Gene Needed for Sex-Lethal Expression in Drosophila Melanogaster
- Multiple response elements in the Sex-lethal early promoter ensure its female-specific expression pattern.
