Terapia com laser em baixa intensidade na prevenção dos efeitos causados pela elevada concentração de glicose na proliferação e migração de fibroblastos / Prevention of high glucose concentration effects in fibroblasts proliferation and migration by low intensity laser therapy.

AUTOR(ES)

Karen Müller Ramalho

DATA DE PUBLICAÇÃO

2007

RESUMO

Diabetic complications, including wound healing deficiency, are usually associated to chronic hyperglycemia, which leads to oxidative stress and aleatory protein glycation. The aim of this study was to evaluate the effects of high glucose concentrations (HGC) on proliferation, migration and death of cultured fibroblasts, as well as the potential effect of low intensity laser therapy (LILT) (660 and 780 nm; 40mW; 2, 5, 10 and 30J/cm2) preventing these effects. HGC reduced cell proliferation in about 40%, cell migration in about 50% and increased cell death in 30%. Daily irradiation at 660nm and 10J/cm2 was efficient preventing the effects on cell proliferation and migration. HGC also increased the production of reactive oxygen species, an effect totally prevented by LILT. The results suggest that LILT favors cell proliferation and migration, processes very important for healing, possibly through an anti-oxidant effect.

ASSUNTO(S)

fibroblasts proliferation diabetes mellitus migração glicose glucose laser laser proliferação fibroblasto migration diabetes mellitus

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