Taxonomic profile of Candida albicans, C. dubliniensis AND C. stellatoidea cultures maintained at urm culture collection, Federal University of Pernambuco / Perfil taxonÃmico das culturas de Candida albicans, C. dubliniensis E C. stellatoidea estocadas na micoteca urm, Universidade Federal de Pernambuco

AUTOR(ES)

Polyanna Nunes Herculano

DATA DE PUBLICAÇÃO

2002

RESUMO

Yeasts consist of a microorganism group with diverse morphology. Similar morphologic and phenotypic characteristics between some fungi difficult the taxonomy of these organisms. Candida species have been identified through morphologic and physiologic characteristics. However, parameters such as assimilation of some composts, cell wall composition and production of extra cellular enzymes may vary between some species. The aims of this research were to confirm taxonomic classification, test the resistance to chemical composts and verify proteolytic activity of Candida albicans, C. dubliniensis and C. stellatoidea cultures maintained at URM Culture Collection, Mycology Department, Federal University of Pernambuco. Tests were conducted to confirm taxonomy through macroscopic, microscopic and physiologic characteristics. The viability of the cultures and preservation of typical characteristics of the species, maintained for 51 years, were verified on 56 isolates. Macroscopic, microscopic and physiologic characteristics confirm the genera but not the species. 29 different resistotypes were obtained among the isolates. About the differentiation of the isolates, all of them were sensitive to malaquite green and resistant to 4-clororesorcinol. The tests permitted to verify that isolates from different and similar Candida species can express variability, through the detection of different resistotypes. Among Candida isolates the proteolytic activity varies from moderate (64%) to absent (36%)

ASSUNTO(S)

resistograma viability confirmaÃÃo taxonÃmica atividade proteolÃtica taxonomic confirmation proteolytic activity candida viabilidade micologia candida resistogram

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