Studies with Detached Lupin Root Nodules in Culture: I. Maintenance and Induction of Acetylene Reduction Activity
AUTOR(ES)
Sutton, William D.
RESUMO
A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr−1 mg−1 fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr−1 mg−1 fresh weight.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541894Documentos Relacionados
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