Nitrogen Metabolism in Soybean Tissue Culture: I. Assimilation of Urea
AUTOR(ES)
Polacco, Joseph Carmine
RESUMO
Cultured soybean (Glycine max, Kanrich variety) cells grow with 25 mm urea as the sole nitrogen source but at a slower rate than with the Murashige and Skoog (MS) (Physiol. Plant. 15: 473-497, 1962) nitrogen source of 18.8 mm KNO3 and 20.6 mm NH4NO3. Growth with urea is restricted by 18.8 mm NO3−, 50 mm methylammonia, 10 mm citrate or 100 μm hydroxyurea, substances which are much less restrictive or nonrestrictive in the presence of ammonia nitrogen source. The restrictive conditions of urea assimilation were examined as possible bases for selection schemes to recover urease-overproducing mutants. Since urease has higher methionine levels than the soybean seed proteins among which it is found, such selections may be a model for improving seed protein quality by plant cell culture techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542245Documentos Relacionados
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