Stimulation of H2S Emission from Pumpkin Leaves by Inhibition of Glutathione Synthesis 1

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The effect of inhibitors of glutathione (GSH) synthesis, namely γ-methyl glutamic acid, d-glutamic acid, cystamine, methionine-S-sulfoximine (MSX), buthionine-S-sulfoximine, and GSH itself, on the emission of H2S was investigated. All these compounds stimulated H2S emission from pumpkin (Cucurbita pepo L. cv Small Sugar Pumpkin) leaf discs in response to sulfate. MSX and GSH were the most effective compounds, stimulating H2S emission from leaf discs of mature pumpkin leaves by about 80% in response to sulfate. Both inhibitors did not appreciably enhance H2S emission in response to l-cysteine and inhibited H2S emission in response to sulfite.

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