Stabilized, Freeze-Dried PCR Mix for Detection of Mycobacteria

AUTOR(ES)

Klatser, Paul R.

FONTE

American Society for Microbiology

RESUMO

We report here the development of a freeze-drying procedure allowing stabilization at ambient temperature of preoptimized, premixed, and predispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4°C and at 20°C for 1 year and for 3 months at 37°C, as judged by their performance with 50 and 500 fg of purified Mycobacterium bovis BCG target DNA.

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