Repair of Injury in Freeze-Dried Salmonella anatum1
AUTOR(ES)
Ray, B.
RESUMO
Repair of injury induced by freeze-drying Salmonella anatum in nonfat milk solids occurred rapidly after rehydration. Injury in surviving cells was defined as the inability to form colonies on a plating medium containing deoxycholate. Death was defined as inability to form colonies in the same medium without this selective agent. The rate of repair of injury was reduced by lowering the temperature from 35 C to 10 C and was extremely low at 1 C. Repair was independent of influence of pH between 6.0 and 7.0. Repair did not require synthesis of protein, ribonucleic acid, or cell wall mucopeptide, but did require energy in the form of adenosine triphosphate (ATP) synthesized through oxidative phosphorylation. The requirement for ATP was based on dinitrophenol or cyanide interference with repair. Dinitrophenol activity was pH-dependent; no repair occurred at pH 6.0 and some repair was observed at pH 6.5 and above. Injured cells were extremely sensitive to low concentrations of ethylenedinitrilotetraacetate. This indicated that freeze-drying injury of S. anatum may involve the lipopolysaccharide portion of the cell wall and that repair of this damage requires ATP synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376322Documentos Relacionados
- Effect of Rehydration on Recovery, Repair, and Growth of Injured Freeze-Dried Salmonella anatum1
- Characterization of the Repair of Injury Induced by Freezing Salmonella anatum1
- Freeze-dried rubella virus hemagglutinating antigen.
- Production of freeze-dried human antihaemophilic cryoprecipitate.
- Influence of Platen Temperatures and Storage Conditions on the Survival of Freeze-dried Salmonella typhimurium1