Sporulation of Bacillus stearothermophilus

AUTOR(ES)

Thompson, P. J.

RESUMO

A broth medium containing tryptone and manganese sulfate supported heavy sporulation of Bacillus stearothermophilus ATCC 7953 (NCA 1518) and four isolates identified as B. stearothermophilus. Maximal spore yields were obtained by use of inocula grown anaerobically in a medium containing glucose with aeration of sporulation medium via bubbling. After an extended stationary period, sporulation occurred concurrently with vegetative growth between 6 and 8 hr of incubation at 60 C. Omission of glucose from the inoculum or use of a “young” (2 hr) inoculum abolished the stationary period, but decreased spore yields. A requirement of oxygen for rapid vegetative growth and sporulation was demonstrated. Manganese (15 to 30 ppm) stimulated sporulation but did not enhance cell growth.

Documentos Relacionados

• Effect of pH on Sporulation of Bacillus Stearothermophilus
• Sporulation and Heat Resistance of Bacillus stearothermophilus Spores Produced in Chemically Defined Media1
• Thermocins of Bacillus stearothermophilus
• Cloning and Expression of Thermostable α-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis
• LIPIDS OF BACILLUS STEAROTHERMOPHILUS, 12