Solid-phase enzyme immunoassay for Chlamydial antibodies.
AUTOR(ES)
Saikku, P
RESUMO
An enzyme immunoassay (EIA) for chlamydial immunoglobulin G antibodies was developed by using microtiter wells coated with partially purified reticulate bodies of Chlamydia trachomatis serotype L2, grown in McCoy cells, and uninfected McCoy cells as a control. Duplicate testing of a single serum dilution, 1:500, was found to be sufficient. A good correlation between positive reactions was observed in a comparative study of 421 patient sera with the EIA and an inclusion immunofluorescence test. A good correlation between positive reactions was also observed in a comparative study of 140 patient sera with EIA and microimmunofluorescence tests in which chlamydial elementary or reticulate bodies were used as antigens. Sera of 77 healthy control individuals with low titers in inclusion immunofluorescence or complement fixation tests gave negative results in the EIA. Immunoblotting experiments showed that the major antigenic component in the EIA antigen was a protein with an Mr of 39,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272567Documentos Relacionados
- Solid-phase enzyme immunoassay for determination of antibodies to cytomegalovirus.
- Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.
- Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.
- Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay.
- Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.
