Sintese do processo de obtenção de dextrana clinica e frutose a partir de sacarose

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

Dextran is a polysaccharid exclusively composed of monomeric units of glucose, joined by a-1,6 glucosidic bonds at the linear position. This polysaccharid is of a great industrial interest at the petrochemistry, food, chemistry and pharmaceutical areas. During the synthesis process of dextran occurs the frutose liberation, that is a monosaccharid of high interest in the food industry. The aim of this work was to synthesize the process steps for clinical dextran and fructose production trom sucrose. The fermentation with Leuconostoc mesenteroides was accomplished in a fed-batch process, for obtaining the extracellular dextran-sucrase enzyme. The dextran was synthesized by the enzyme using sucrose as substrate at the concentrations of 50 and 1OOgIL. The clínical dextran was obtained by acid hydrolysis of the crude dextran produced at the synthesis and further separation with different ethanol concentrations. The process yields for the dextran with high molecular and fructose obtained from maximum theoretical value was: conventional process - 24,9 and 42,2%, enzymatic process with unmanufactered enzyme - 24,9 and 55,7% and enzymatic process with purified enzyme - 41,6 and 80,2%. On enzymatic process with purified enzyme, percentage of dextran of high molecular mass obtained was: 70,4 and 55,5% for sucrose concentration of 50 g/L and 1OOg/L, respectively. On acid hydrolysis, the best results were obtained with temperature of 76°C and pH 1,0, in process time of 4,5 hours with results it can conclude that the best process for obtaining clinical dextran and fructose was enzymatic synthesis with purified enzyme

ASSUNTO(S)

frutose sacarose

ACESSO AO ARTIGO

Documentos Relacionados