Desenvolvimento de processo continuo de obtenção de frutose a partir de sacarose. / Development of continuous process of attainment of frutose from sacarose.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The consumption of fructose has increased in the last few years, as this sugar is being more and more used as a substitute to sucrose, due to the fisiological benefits pointed out by recent research. Between many methods to obtain high-purity fructose starting from sucrose, the utilization of immobilized enzymes in biorrectors presents advantages, as the utilization of mild operational condition and low sub-product formation. The resulting mixture of glucose-fructose can be separated by low-cost chromatographic techniches, as the utilization of zeolyt as adsorbent. The objective of this study was to develop a process to the attainment of fructose starting from sucrose. The hidrolysys of sucrose was studied in a fixed bed containing the inulinase enzyme immobilized and also, after that, the separation of glucose and fructose in an adsorption column containing Y zeolite. At first, it was carried out the characterization of the inulinase of Kluyveromyces marxianus var. bulgaricus immobilized in calcium alginate with the addition of actived carbon and it was observed that the half-life of the enzyme is increased, and at 50oC it is 11,1 days, which shows good perspectives of industrial utilization. The ideal process temperature for the biorreactor is 43oC. With the pH fixed in 4,8, the sucrose convertion was studied in a fixed bed containing 105 mL of immobilized inulinase and a residence time of 6,2 hours. In these conditions, it was obtained 98% of convertion, and the half-life of the reactor experimentally obtained was 74 days. The study of separation of glucose and fructose in a bed containing Y zeolite in baric form showed that the best condition for this separation was 3 series columns with a total volume of 117,9 cm3 and ethanol 15% as desorbent, resulting in 1,62 of separation efficiency. In these conditions, it was recovered 63,5% of the total fructose injected. For the global process, comprising the sucrose hidrolysys and fructose and glucose separation, it was designed 3 columns for the separation, as the hidrolysys is the limiting stage. The columns must have 75 cm height, 5,66 cm of diameter and a flow rate of 3,27 mL/min. The desorbent used was ethanol 15%.

ASSUNTO(S)

zeolites frutose zeolitos separação (tecnologia) frutose separation (technology) hydrolysis hidrolise

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