Sequencing and analysis of a EST Bank from salivary glands of Rhynchosciara americana and characterization of the gene RaDup / Sequenciamento e análise de um banco de cDNA de glândulas salivares de Rhynchosciara americana e caracterização do gene RaDup

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

In this work EST sequencing was used as strategy to find messages related to development, metabolism and polyteny/amplification in salivary glands of Rhynchosciara americana, a dipteran (Sciaridae) that shows in this tissue giant polytene chromosomes and gene amplification tightly regulated throughout development of the larvae. A total of 8193 EST sequences were generated, annotated and categorized using Gene Ontology Consortium terms, providing a general view of the metabolic status, like an electronic Northern, of an important point in development of the larvae, that shows where specific genes are amplified and the salivary gland needs to secrete the proteins to form the cocoon. Other data include determination of 91 SNPs and a statistic of codon usage. Several ESTs were identified with potential connection to endocicles, from these RaDup and RaMCM5 were selected for further studies. Both chromosomal loci were identified and genomic regions isolated, for RaDup the coding region of its mRNA and 12kb of genomic region were completely sequenced, revealing its gene structure, and antibodies were raised against this protein, making evident data about its involvement in replication in mitotic cells and in endocicles in salivary glands. About the chromosomal locus of RaDup, it becomes very interesting, because for the first time one amplified puff can be related to a regulatory gene.

ASSUNTO(S)

gene amplification rhynchosciara ciclo celular dipteros amplificação de genes politene puff endociclos rhynchosciara cell cycles endocycle politenia sciarídeos dipteran sciaridae

ACESSO AO ARTIGO

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