Gene Amplification
Mostrando 1-12 de 2915 artigos, teses e dissertações.
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1. Characterization of Phytophthora nicotianae isolates from tobacco plants (Nicotiana tabacum) in Colombia
ABSTRACT The black shank disease caused by Phytophthora nicotianae causes losses in tobacco crops up 100%. In Colombia, P. nicotianae populations are poorly known causing wrong diagnostics and erratic management. Amplification of the Ypt1 gene and morphological characteristics of colonies, sporangia, chlamydospores and hyphae were used to identify P. nicotia
Revista Ceres. Publicado em: 2022
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2. Screening and identification of bacteriocin-like inhibitory substances producing lactic acid bacteria from fermented products
Abstract A total of 544 isolates of lactic acid bacteria isolated from fermented products: 451 isolates from fermented products from Thailand and 93 isolated from French fermented products were screened for bacteriocin production. 10 isolates showed antimicrobial activities. Identification of the selected strains by random amplification of polymorphic DNA-po
Food Sci. Technol. Publicado em: 2020-09
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3. Screening and identification of bacteriocin-like inhibitory substances producing lactic acid bacteria from fermented products
Abstract A total of 544 isolates of lactic acid bacteria isolated from fermented products: 451 isolates from fermented products from Thailand and 93 isolated from French fermented products were screened for bacteriocin production. 10 isolates showed antimicrobial activities. Identification of the selected strains by random amplification of polymorphic DNA-po
Food Sci. Technol. Publicado em: 20/12/2019
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4. Emerging carbapenem-resistant Aeromonas spp. infections in Cali, Colombia
ABSTRACT Introduction: Aeromonas species are renowned enteric pathogens with virulence determinants linked to human diseases, such as gastroenteritis, skin, soft-tissue and muscle infections, and septicemia. A recent concern of resistance in this organism has emerged, especially the presence carbapenemases. Herein we describe a case series of emerging carba
Braz J Infect Dis. Publicado em: 25/11/2019
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5. Analysis on genetic diversity of 51 Grape germplasm resources
RESUMO: O objetivo deste estudo é investigar a diversidade genética da uva ‘Zuijinxiang’ e de suas plantas F1 reprodutoras mutantes. Foram selecionadas as melhores plantas mutantes com potencial e valor genético. Utilizaram-se como objeto de pesquisa 50 plantas individuais mutantes obtidas de sementes de uva irradiadas com 137Cs-γ ‘Zuijinxiang’,
Cienc. Rural. Publicado em: 21/10/2019
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6. TIM-3 genetic variants and risk of Behçet disease in the Iranian population
Abstract: Background: Behçet disease is a prototypical systemic autoimmune disease, caused by a complex interplay between environmental and genetic factors. The transmembrane immunoglobulin and mucin domain-3 (TIM-3) is a distinct member of the TIM family that is preferentially expressed on Th1 cells and plays a role in Th1-mediated autoimmune or inflammat
An. Bras. Dermatol.. Publicado em: 17/10/2019
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7. Improvement of the specific detection of Xanthomonas phaseoli pv. manihotis based on the pthB gene
ABSTRACT. Modifications were made in the PCR conditions aiming to overcome the problem of non-amplification of the Xanthomonas phaseoli pv. manihotis (Xpm) fragment, using the primer pair XV / XK described in the literature. The objective of this study was to propose changes in the primers already described (XV / XK_MOD) and validate the use of these new pri
Acta Sci., Agron.. Publicado em: 23/09/2019
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8. MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON STAPHYLOCOCCUS SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE
Resumo O presente trabalho objetivou avaliar a eficácia in vitro de desinfetantes comerciais frente à Staphylococcus spp. isolados de casos de mastite em cabras e, relacionar a resistência observada com a presença do gene icaD. A microdiluição em caldo foi utilizada para avaliar a atividade antimicrobiana in vitro dos desinfetantes, enquanto a técnica
Ciênc. anim. bras.. Publicado em: 22/08/2019
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9. Isolation and characterization of hydrocarbon-degrading bacteria from gas station leaking-contaminated groundwater in the Southern Amazon, Brazil
Resumo Vinte e três linhagens bacterianas degradadoras de hidrocarbonetos foram isoladas de água subterrânea contaminada por vazamento em posto de combustível no sul da Amazônia, Brasil. Com base na habilidade de degradar hidrocarbonetos (diesel, hexadecano, benzeno, tolueno e xileno), duas linhagens foram selecionadas para estudos posteriores. A amplif
Braz. J. Biol.. Publicado em: 05/08/2019
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10. Clinical and molecular profile of newborns with confirmed or suspicious congenital adrenal hyperplasia detected after a public screening program implementation,
Resumo Objetivo: Descrever os resultados obtidos em um programa de triagem neonatal após sua implementação e avaliar os perfis clínicos e moleculares de casos confirmados e suspeitos de hiperplasia adrenal congênita. Métodos: Foi feito um estudo transversal. Recém-nascidos com suspeita da doença devido aos altos níveis de 17-alfa-hidroxiprogestero
J. Pediatr. (Rio J.). Publicado em: 01/07/2019
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11. Searching for mutations in the HNF1B gene in a Brazilian cohort with renal cysts and hyperglycemia
ABSTRACT Objective To verify the presence of variants in HNF1B in a sample of the Brazilian population selected according to the presence of renal cysts associated with hyperglycemia. Subjects and methods We evaluated 28 unrelated patients with clinical suspicion of HNF1B mutation because of the concomitant presence of diabetes mellitus (DM) or prediabet
Arch. Endocrinol. Metab.. Publicado em: 02/05/2019
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12. Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil
RESUMO: Toxina A de Clostridium chauvoei (CctA), neuraminidase (NanA) e flagelina (FliC) são proteínas que contribuem para a patogenicidade de Clostridium chauvoei, o agente causador do carbúnculo sintomático em bovinos. O objetivo deste estudo foi analisar a variabilidade genética dos genes cctA, nanA, e fliC em C. chauvoei isolados em diferentes per�
Cienc. Rural. Publicado em: 02/05/2019