Screening and selection of wild strains for L-arabinose isomerase production
AUTOR(ES)
Manzo, R. M., Simonetta, A. C., Rubiolo, A. C., Mammarella, E. J.
FONTE
Braz. J. Chem. Eng.
DATA DE PUBLICAÇÃO
2013-12
RESUMO
The majority of L-arabinose isomerases have been isolated by recombinant techniques, but this methodology implies a reduced technological application. For this reason, 29 bacterial strains, some of them previously characterized as L-arabinose isomerase producers, were assayed as L-arabinose fermenting strains by employing conveniently designed culture media with 0.5% (w/v) L-arabinose as main carbon source. From all evaluated bacterial strains, Enterococcus faecium DBFIQ ID: E36, Enterococcus faecium DBFIQ ID: ETW4 and Pediococcus acidilactici ATCC ID: 8042 were, in this order, the best L-arabinose fermenting strains. Afterwards, to assay L-arabinose metabolization and L-arabinose isomerase activity, cell-free extract and saline precipitated cell-free extract of the three bacterial cultures were obtained and the production of ketoses was determined by the cysteine carbazole sulfuric acid method. Results showed that the greater the L-arabinose metabolization ability, the higher the enzymatic activity achieved, so Enterococcus faecium DBFIQ ID: E36 was selected to continue with production, purification and characterization studies. This work thus describes a simple microbiological method for the selection of L-arabinose fermenting bacteria for the potential production of the enzyme L-arabinose isomerase.
Documentos Relacionados
- l-Ribose Production from l-Arabinose by Using Purified l-Arabinose Isomerase and Mannose-6-Phosphate Isomerase from Geobacillus thermodenitrificans▿
- l-Arabinose Negative Mutants of the l-Ribulokinase Structural Gene Affecting the Levels of l-Arabinose Isomerase in Escherichia Coli
- Induction and Repression of l-Arabinose Isomerase in Salmonella typhimurium
- INDUCTION AND REPRESSION OF l-ARABINOSE ISOMERASE IN PEDIOCOCCUS PENTOSACEUS1
- Induction and Repression of l-Arabinose Isomerase in Bacteriophage-Infected Salmonella typhimurium
