INDUCTION AND REPRESSION OF l-ARABINOSE ISOMERASE IN PEDIOCOCCUS PENTOSACEUS1
AUTOR(ES)
Dobrogosz, Walter J.
RESUMO
Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Induction and repression of l-arabinose isomerase in Pediococcus pentosaceus. J. Bacteriol. 85:1350–1355. 1963.—The inducible l-arabinose isomerase of Pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. The enzyme is measured by its ability to catalyze the isomerization of l-arabinose to l-ribulose. Whole cells suspended in a suitable buffer and pretreated with toluene were shown to exhibit this isomerase activity at a level comparable with that observed in cell-free enzyme preparations. Conditions for optimal induction of l-arabinose isomerase are described. In addition, it was determined that the formation of this enzyme is subject to repression by glucose, i.e., via catabolite repression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=278340Documentos Relacionados
- Induction and Repression of l-Arabinose Isomerase in Salmonella typhimurium
- Induction and Repression of l-Arabinose Isomerase in Bacteriophage-Infected Salmonella typhimurium
- l-Ribose Production from l-Arabinose by Using Purified l-Arabinose Isomerase and Mannose-6-Phosphate Isomerase from Geobacillus thermodenitrificans▿
- l-Arabinose Negative Mutants of the l-Ribulokinase Structural Gene Affecting the Levels of l-Arabinose Isomerase in Escherichia Coli
- Screening and selection of wild strains for L-arabinose isomerase production
