Role of proton motive force in genetic transformation of Bacillus subtilis.
AUTOR(ES)
van Nieuwenhoven, M H
RESUMO
This study explored the role of the proton motive force in the processes of DNA binding and DNA transport of genetic transformation of Bacillus subtilis 168 strain 8G-5 (trpC2). Transformation was severely inhibited by the ionophores valinomycin, nigericin, and 3,5-di-tert-4-hydroxybenzylidenemalononitrite (SF-6847) and by tetraphenylphosphonium. The ionophores valinomycin and nigericin also severely inhibited binding of transforming DNA to the cell envelope, whereas SF-6847 and carbonylcyanide-p-trifluoromethoxyphenylhydrazone hardly affected binding. The proton motive force, therefore, does not contribute to the process of DNA binding, and valinomycin and nigericin interact directly with the DNA binding sites at the cell envelope. The effects of ionophores, weak acids, and tetraphenylphosphonium on the components of the proton motive force and on the entry of transforming DNA after binding to the cell envelope was investigated. DNA entry, as measured by the amount of DNase I-resistant cell-associated [3H]DNA and by the formation of DNA breakdown products, was severely inhibited under conditions of a small proton motive force and also under conditions of a small delta pH and a high electrical potential. These results suggest that the proton motive force and especially the delta pH component functions as a driving force for DNA uptake in transformation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=220324Documentos Relacionados
- Quantitative measurements of proton motive force and motility in Bacillus subtilis.
- Proton motive force may regulate cell wall-associated enzymes of Bacillus subtilis.
- Characterization of the Bacillus subtilis motile system driven by an artificially created proton motive force.
- The membrane-induced proton motive force influences the metal binding ability of Bacillus subtilis cell walls.
- Genetic transformation with cell wall-associated deoxyribonucleic acid in Bacillus subtilis.