Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
AUTOR(ES)
Richini-Pereira, VB, Bosco, SMG, Theodoro, RC, Barrozo, L, Bagagli, E
FONTE
Journal of Venomous Animals and Toxins including Tropical Diseases
DATA DE PUBLICAÇÃO
2010
RESUMO
Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.
Documentos Relacionados
- Molecular approaches for eco-epidemiological studies of Paracoccidioides brasiliensis
- Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
- Human leptospirosis in the Federal District, Brazil, 2011-2015: eco-epidemiological characterization
- Physical environment and life expectancy at birth in Mexico: an eco-epidemiological study
- Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of São Paulo, Brazil