Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil
AUTOR(ES)
Richini-Pereira, Virginia Bodelão, Marson, Pamela Merlo, Hayasaka, Enio Yoshinori, Victoria, Cassiano, Silva, Rodrigo Costa da, Langoni, Hélio
FONTE
J. Venom. Anim. Toxins incl. Trop. Dis
DATA DE PUBLICAÇÃO
05/08/2014
RESUMO
Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmania spp., offering new opportunities for epidemiological studies of this infection. Methods This study aimed to evaluate the presence of Leishmania spp. and Leishmania chagasi DNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals. Results DNA was detected in tissues of one Cavia aperea (Brazilian guinea pig), five Cerdocyon thous(crab-eating fox), one Dasypus septemcinctus (seven-banded armadillo), two Didelphis albiventris(white-eared opossum), one Hydrochoerus hydrochoeris (capybara), two Myrmecophaga tridactyla(giant anteater), one Procyon cancrivorus (crab-eating raccoon), two Sphiggurus spinosus(porcupine) and one Tamandua tetradactyla (lesser anteater) from different locations in the Central Western part of São Paulo state. The Leishmania chagasi DNA were confirmed in mesenteric lymph node of one Cerdocyon thous. Results indicated common infection in wild animals. Conclusions The approach employed herein proved useful for detecting the environmental occurrence ofLeishmania spp. and L. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction.
Documentos Relacionados
- Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of São Paulo, Brazil
- Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens
- Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil
- The structural molecular biology network of the State of São Paulo, Brazil
- The interface between wild small mammals and domestic animals of the fragmented area from High Paranapanema, Sao Paulo state, Brazil