Molecular detection of Leishmania spp. in road-killed wild mammals in the Central Western area of the State of São Paulo, Brazil

AUTOR(ES)
FONTE

J. Venom. Anim. Toxins incl. Trop. Dis

DATA DE PUBLICAÇÃO

05/08/2014

RESUMO

Background Road-killed wild animals have been classified as sentinels for detecting such zoonotic pathogens asLeishmania spp., offering new opportunities for epidemiological studies of this infection. Methods This study aimed to evaluate the presence of Leishmania spp. and Leishmania chagasi DNA by PCR in tissue samples (lung, liver, spleen, kidney, heart, mesenteric lymph node and adrenal gland) from 70 road-killed wild animals. Results DNA was detected in tissues of one Cavia aperea (Brazilian guinea pig), five Cerdocyon thous(crab-eating fox), one Dasypus septemcinctus (seven-banded armadillo), two Didelphis albiventris(white-eared opossum), one Hydrochoerus hydrochoeris (capybara), two Myrmecophaga tridactyla(giant anteater), one Procyon cancrivorus (crab-eating raccoon), two Sphiggurus spinosus(porcupine) and one Tamandua tetradactyla (lesser anteater) from different locations in the Central Western part of São Paulo state. The Leishmania chagasi DNA were confirmed in mesenteric lymph node of one Cerdocyon thous. Results indicated common infection in wild animals. Conclusions The approach employed herein proved useful for detecting the environmental occurrence ofLeishmania spp. and L. chagasi, as well as determining natural wild reservoirs and contributing to understand the host-parasite interaction.

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