Rapid Identification, Differentiation, and Proposed New Taxonomic Classification of Bifidobacterium lactis

AUTOR(ES)

Ventura, Marco

FONTE

American Society for Microbiology

RESUMO

Identification of Bifidobacterium lactis and Bifidobacterium animalis is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. Analysis of the 16S-23S internally transcribed spacer region of B. lactis DSM10140T, B. animalis ATCC 25527T, and six potential B. lactis strains suggested two distinct clusters. Two specific 16S-23S spacer rRNA gene-targeted primers have been developed for specific detection of B. animalis. All of the molecular techniques used (B. lactis or B. animalis PCR primers, enterobacterial repetitive intergenic consensus PCR) demonstrated that B. lactis and B. animalis form two main groups and suggest a revision of the strains assigned to B. animalis. We propose that B. lactis should be separated from B. animalis at the subspecies level.

Documentos Relacionados

• Sugarcane phytocystatins: Identification, classification and expression pattern analysis
• Classification, Identification, and Clinical Significance of Proteus, Providencia, and Morganella
• Identification, classification and expression pattern analysis of sugarcane cysteine proteinases
• Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene
• Methodological parameters for the identification and taxonomic classification of isolated theropodomorph teeth