Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR

AUTOR(ES)

Kurupati, Prathiba

FONTE

American Society for Microbiology

RESUMO

A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to results of conventional biochemical characterization. The assay also showed 100% sensitivity, as it correctly identified all 142 positive-control blood cultures and 4 from double-blind trials.

Documentos Relacionados

• Quantitative Detection of Respiratory Chlamydia pneumoniae Infection by Real-Time PCR
• Rapid Detection of Clostridium difficile in Feces by Real-Time PCR
• Quantitative Detection of Streptococcus pneumoniae in Nasopharyngeal Secretions by Real-Time PCR
• Identification of Histoplasma capsulatum from Culture Extracts by Real-Time PCR
• Rapid Quantitative Detection of Listeria monocytogenes in Meat Products by Real-Time PCR