Rapid Quantitative Detection of Listeria monocytogenes in Meat Products by Real-Time PCR
AUTOR(ES)
Rodríguez-Lázaro, David
FONTE
American Society for Microbiology
RESUMO
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522080Documentos Relacionados
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