Rapid activation by interferon alpha of a latent DNA-binding protein present in the cytoplasm of untreated cells.
AUTOR(ES)
Dale, T C
RESUMO
The highly conserved interferon (IFN)-stimulated regulatory elements of the human genes 6-16 and 9-27 bind to one or more proteins (E factor) detected in extracts of human Bristol 8 B cells or human foreskin fibroblast cells treated with IFN-alpha. E factor is not detectable in extracts of untreated cells and appears in IFN-treated cells within less than 1 min in a form extractable with low salt and thus presumably not bound to DNA. After a few more minutes, the level of this form decreases in parallel with the increase of a form extractable only with high salt and thus presumably bound to DNA. Induction of E factor by IFN-alpha can occur in nuclei-free cytoplasts, whereas no E factor was detected in IFN-treated nucleoplasts. Together, these results suggest a model for signal transduction in which latent E factor, located in the cytoplasm, is activated or released from an inhibitor very rapidly upon binding of IFN-alpha to its receptor. Active E factor can then migrate to the nucleus, where it binds to the IFN-stimulated regulatory elements of IFN-regulated genes, activating their transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=286654Documentos Relacionados
- DNA-binding proteins present in varicella-zoster virus-infected cells.
- DNA-binding protein activated by gamma radiation in human cells.
- DNA-binding proteins in the cytoplasm of vaccinia virus-infected mouse L-cells.
- UV-induced DNA-binding proteins in human cells.
- Rapid isolation of specific DNA-binding proteins and their DNA-binding domains.