Purificação e caracterização de uma ß-N-acetillhexosaminadase extraída do mamífero marinho Sotalia fluviatilis

AUTOR(ES)

José Edilson Gomes Júnior

DATA DE PUBLICAÇÃO

2006

RESUMO

This report shows 2232 times purification of a βNAcetylhexosaminidase from hepatic extracts from the sea mammal Sotalia fluviatilis homogenate with final recovery of 8,4%. Sequenced steps were utilized for enzyme purification: ammonium sulfate fractionation, Biogel A 1.5 m, chitin, DEAESepharose and hydroxyapatite chromatographies. The protein molecular mass was estimated in 10 kDa using SDSPAGE and confirmed by MALDITOF. It was found to have an optimal pH of 5.0 and a temperature of 60C. Using pnitrophenylNAcetylβDglycosaminide apparent Km and Vmax values were of 2.72 mM and 0.572 nmol/mg/min, respectively. The enzyme was inhibited by mercury chloride (HgCl2) and sodium dodecil sulfate (SDS)

ASSUNTO(S)

sotalia fluviatilis sea mammal β β sotalia fluviatilis glycosaminoglycans nacetilhexosaminidase nacetilglucosaminidase β nacetilgalactosaminidase extratos hepáticos mamíferos marinhos nacetylhexosaminidase hepatics extracts nacetylgalactosaminidase glicosaminoglicanos β β bioquimica β nacetylglycosaminidase

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