Prospecção de processos biotecnológicos de interesse industrial / Prospection of biotechnological processes of industrial interest
AUTOR(ES)
Gustavo Molina
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
03/09/2010
RESUMO
The objective of the present work was to study the biotransformation of monoterpenes to obtain natural flavor compounds (bioflavors), focusing the biochemical processes involved in the procedures investigated for possible industrial applications. Therefore, the study started with 70 strains, isolated in a previous study from a citrus processing plant and capable of use R-(+)-limonene as sole carbon and energy source. These microorganisms were tested for their ability to biotransform this monoterpene on flavor compounds. Among the strains potentially degrading of R-(+)-limonene, just the strain LB285JLB showed significant accumulation of interest metabolite concentration which could justify optimization studies. Subsequently, the Response Surface Methodology was employed to optimize the main parameters of the process of LB285JLB biomass production, using glycerol as carbon source. Only three (glycerol concentration, temperature and agitation) of all parameters tested influenced significantly (p <0.1) the biomass production. TheT optimization of these variables applying a Central Composite Design revealed that the optimal biotrasformation conditions were 6-9g.L-1, 35-40 °C and 230-300 rpm. Finally, preliminary propection studies evaluating the potential of endophytes isolated from Baru (Dipteryx alata Vog.), for the development of biotechnological processes of industrial interest. To date, the studies conducted have shown that endophytic microorganisms may have potential application in biotransformation process for bioflavors production, and also should be considered with process of enzymes co-production. These results encourages that further researchs should be considered to determine the potential of these microorganisms
ASSUNTO(S)
biotransformação bioaromas terpenos prospecção endofíticos biotransformation bioflavors terpenes propspection endophytics
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=000774289Documentos Relacionados
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