Prospecção de bradicininas de anfíbios do gênero Phyllomedusa

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Amphibian skin has a crucial role on the survival and on the capacity of the animal of exploring the most different ambients, That is the reason why they are capable of producing the plethora of compounds, described as defensive mechanisms to avoid pathogen invasion or ingestion by predators. One of these compounds, known as bradykinin, is a nonapeptide originated from the kallicrein-kinin system and may be related to an anti-predation mechanism against the ingestion by predators and that may be the reason that they might have geographic variation allowing an adaptation of amphibians to the ambient where they live. In mammals, bradykinin can cause various physiological effects such as vasodilatation, contraction of smooth muscle and mediate inflammation. The present study describes the molecular screening of BK analogs found in the secretion of three Phyllomedusa genus species from two regions of Brazil. The skin secretion was obtained from adult frog specimens by mild electric stimulation and the crude secretion was submitted to RP-HPLC using semipreparative columns. Novel bradykinin related peptides were described as well as 18 peptides already characterized in the literature. Selected peptides were synthesized and submitted to biological assays in order to assess their mode of action during inflammation and mitogenic processes.

ASSUNTO(S)

phyllmedusa biodicinina espectrometria de massa ciencias biologicas

ACESSO AO ARTIGO

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