Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process
AUTOR(ES)
Singh, Ranjan, Gaur, Rajeeva, Tiwari, Soni, Gaur, Manogya Kumar
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2012-09
RESUMO
Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.
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