Production of Interferon in Serum-Free Human Leukocyte Suspensions1

AUTOR(ES)

Strander, H.

RESUMO

The recovery of interferon from Sendai-infected suspensions of purified human leukocytes is dependent on the serum concentration in the incubation medium. Very little interferon is obtained from serum-free suspensions. The data reported demonstrate that the critical macromolecular, age-independent, and species-unspecific serum principle can be omitted from the suspensions if the medium is supplemented with a combination of crystalline serum albumin and high concentrations of any one of five studied dipolar ionic buffers [N,N-bis(2-hydroxyethyl) glycine (Bicine), N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 2-(N-morpholino)ethanesulfonic acid (MES), N-tris(hydroxymethyl)methyl-2-amino-ethanesulfonic acid (TES), and N-tris(hydroxymethyl)methylglycine (Tricine)]. The optimal combination [TES (1.0%, w/v) and bovine serum albumin (0.8%, w/v)] allows the production of potent preparations of serum-free human interferon.

Documentos Relacionados

• Production of human interferon-gamma in serum-free medium.
• Hormonal control of human colon carcinoma cell growth in serum-free medium.
• Growth of embryonal carcinoma cells in serum-free medium.
• Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
• Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium.