Preparação e caracterização de lipossomas para a administração por inalação de compostos terapeuticos utilizados na terapia da tuberculose

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

Nowadays, Tuberculosis, is one of the most serious problems of public helth worldwide, specially in development countries, being associated to the death of at least three million people yearly. In this context, the present work aimed the preparation nd characterization of liposomes (lipid vesicles) designed to increase the effectiveness of drugs frequently used in Tuberculosis primary and secondary therapy, through the controlled release of these drugs by inhalation. Unilamellar liposomes were prepared with 60% mol of distearoylpHosHatidylcoline and 40% mol of cholesterol. The drugs Isoniazid, Pyrazinamide, Rifampin and Ethionamide were incorporated in the liposomes by passive loading in vesicle aqueous core or in its lipid bilayer. The formulations obtained were characterized in terms of their final lipid and drug concentrations, vesicle mean diameter, and stability during storage at 5 DEGREES C as an aqueous suspension. The sample presenting the best encapsulation efficiencies and storage stability were also characterized concerning to their stability when in contact with the non-ionic surfactant C IND. 12 E IND. 5 . Similar encapsulation studies were also accomplished for the same drugs, besides Streptomycin, using the active loading technique based on imposing a transmembrane pH gradient of 3.4 units. The results indicated a strong association among the experimental protocols, the trapping efficiency and the stability of the obtained systems... Note: The complete abstract is available with the full electronic digital thesis or dissertations

ASSUNTO(S)

tuberculose lipossomos membranas (tecnologia) microencapsulação

ACESSO AO ARTIGO

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