Polymorphic information content of SSR markers for Coffea spp.

AUTOR(ES)
FONTE

CROP BREEDING AND APPLIED BIOTECHNOLOGY

DATA DE PUBLICAÇÃO

2011

RESUMO

Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.

ASSUNTO(S)

ssr arabica coffee molecular mrkers genomic dna libraries enriched

Documentos Relacionados