Poly(L-co-D,L lactic acid-co-trimethylene carbonate) scaffolds for growth of osteoblastic cells (SaOS-2) / Arcabouços de poli(L-co-D,L ácido láctico-co-trimetileno carbonato) para o crescimento de células osteoblásticas (SaOS-2)

AUTOR(ES)

André Dutra Messias

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

30/06/2011

RESUMO

Lactic acid based polymers are widely investigated as materials for tissue engineering. The ability to allow hydrolytic degradation, to be reabsorbed by the body and its inherent biocompatibility, make them an excellent choice for this application. However, characteristics such as low flexibility and low elongation ability before the fracture tend to limit these devices in particular applications, which can be enhanced with the addition of compounds that act as plasticizers, as the trimethylene carbonate (TMC) when present in the polymer chain of the copolymer of lactic acid (PLDLA). The objective of this work was to synthesize and characterize the terpolymer of L-lactic acid, D,L-lactic acid and TMC, obtain evaluation of scaffolds for cell viability and alkaline phosphatase activity of osteoblastic cells (SaOS-2) in 1, 3, 7, 14 and 21 days of culture, aiming its application in bone tissue engineering. Thus, the terpolymer is synthesized from 20 and 30% of TMC, by bulk polymerization of monomers, confirmed by 1H and 13C RMN. The GPC analysis showed that the copolymers synthesized showed average molar mass (Mw) in the order of 105 g/mol, an important feature that allows the attainment of minimum mechanical properties necessary for structural applications. The thermal investigation of thermal PLDLA-TMC showed a slight decrease of Tg comparing to PLDLA. Furthermore, the one step terpolymer degradation was initiated around 290 ºC, as shown by TGA. The MTT assay showed that the PLDLA-TMC scaffolds enabled an increase in cell viability, reaching a peak in 7 days, and remained stable until 14 days of cultivation. Similarly, the alkaline phosphatase activity was increased up to 7 days of culture, an important indicator of osteoblastic activity. These results show that it was possible to successfully produce a terpolymer from L-lactic acid, D,L-lactic acid and trimethylene carbonate. In addition, the scaffolds exhibited important characteristics considering the application for bone tissue engineering.

ASSUNTO(S)

biomateriais polímeros engenharia tecidual osteoblastos biomaterials polymers tissue engineering osteoblasts

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