Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures
AUTOR(ES)
Dempsey, Patrick M.
RESUMO
A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 μM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=186939Documentos Relacionados
- An Alcohol Oxidation System in Streptococci Which Functions without Hydrogen Peroxide Accumulation
- Hydrogen peroxide excretion by oral streptococci and effect of lactoperoxidase-thiocyanate-hydrogen peroxide.
- Hydrogen Peroxide Metabolism in Yeasts
- Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola.
- Antibacterial activity of hydrogen peroxide and the lactoperoxidase-hydrogen peroxide-thiocyanate system against oral streptococci.
