Phytochrome Modification and Light-enhanced, In Vivo-induced Phytochrome Pelletability 12

AUTOR(ES)

Boeshore, Maury L.

RESUMO

Phytochrome that was induced by red irradiation in vivo to pellet with subcellular material and that was released from the pellet by removal of divalent cations exhibited altered characteristics. Compared to phytochrome extracted in a soluble red-absorbing form from etiolated tissue, pelleted and released phytochrome, which was also assayed in the red-absorbing form even though pelleted in the far-red-absorbing form, showed 50% greater micro complement fixation activity, eluted closer to the void volume of a Sephadex G-200 column, and electrophoresed more slowly on sodium dodecyl sulfate-polyacrylamide gels. Data presented here document that phytochrome pelleted in the far-red-absorbing form differs from soluble phytochrome extracted from nonirradiated tissue. These data, however, do not permit the conclusion that there is a causal relationship between pelletability and phytochrome modification.

Documentos Relacionados

• Red Light-enhanced Phytochrome Pelletability: Re-examination and Further Characterization 1
• In Vivo-Induced Genes in Pseudomonas aeruginosa
• Identification of Listeria monocytogenes In Vivo-Induced Genes by Fluorescence-Activated Cell Sorting
• Phytochrome Pelletability Induced by Irradiation in Vivo: MIXING EXPERIMENTS 1
• ohr, Encoding an Organic Hydroperoxide Reductase, Is an In Vivo-Induced Gene in Actinobacillus pleuropneumoniae