Particle Movements in Chloroplast Membranes: Quantitative Measurements of Membrane Fluidity by the Freeze-Fracture Technique

AUTOR(ES)

Ojakian, George K.

RESUMO

Stacked chloroplast membranes isolated from Chlamydomonas reinhardtii have differentiated particle arrays when examined by freeze-fracture electron microscopy. When the membranes are isolated unstacked, these particle arrays are lost and the fracture faces have a homogeneous appearance. The changes in appearance are due to rearrangement of existing membrane components by lateral particle movements in the plane of the fluid chloroplast membranes, since quantitative measurements demonstrate almost complete conservation of numbers and sizes of membrane particles during experimentally controlled stacking and unstacking.

Documentos Relacionados

• Particle movements in chloroplast membranes: Quantitative measurements of membrane fluidity by the freeze-fracture technique
• A structural change of the plasma membrane induced by oncogenic viruses: quantitative studies with the freeze-fracture technique.
• Fracture-label:O cytochemistry of freeze-fracture faces in the erythrocyte membrane.
• Changes in the organization of the outer membrane of Proteus mirabilis during swarming: freeze-fracture structure and membrane fluidity analysis.
• Analysis of Borrelia burgdorferi membrane architecture by freeze-fracture electron microscopy.