Participation of galectin-1 in the evolution of experimental histoplasmosis / Participação da galectina-1 na evolução da histoplasmose experimental
AUTOR(ES)
Lilian Cataldi Rodrigues
DATA DE PUBLICAÇÃO
2007
RESUMO
Galectin-1 (Gal-1) belongs an endogenous lectins family that recognizes -galactoside and participates of various biological activities. This lectin can modulate the innate and adaptative immune responses. Although, Gal-1 participates of various pathophysiological processes, in literature we did not find reports related to the participation of Gal-1 in fungal infections. The aim of this work was to investigate the biological impact of Gal-1 in the experimental histoplasmosis. The mice (GAL-1-/- and GAL-1+/+) were injected (i.t.) with 5 x 105 yeast cell and at 15 days post-infection, BALF cells and lungs cytokine and PGE2 were measured by ELISA. The Recovery of H. capsulatum was made in lung and spleen and the fungal burden was assessed as the CFU per organ. The lung slices were stained by hematoxiline eosin or with Gomoris methanemine silver (GMS) and submitted to histopathological investigation and quantification of neutrophil or fungus, respectively. Total and differential cell counts of the bronchoalveolar lavage (BAL).were performed using diluting solution in Neubauer chamber and Rosenfeld-stained smear. The capacity of Gal-1 to induce or modulate the lipids bodies formation by fungus components was analyzed by staining treated cells with osmium tetroxide. The total nitrite (NO2) concentration in the animals serum was measured by Griess reaction. All H. capsulatum-infected wild type mice survived until 30 days post-infection, whereas only 33% of the Gal-1-/- infected mice died during of this period of observation. At 15 days post-infection, CFU were found to be higher in the spleens or lung from infected-Gal1-/- mice. The number of neutrophils in the lung of the infected-Gal-1-/- mice higher than infected Gal-1+/+ animals. Curiously, H. capsulatum infected Gal-1-/- mice, presented higher levels of PGE2 and TH1 inflammatory cytokines (IFN-, IL-1 e IL-12) in comparison with wild type infected-mice. Adherent peritoneal cells peritoneal derived from Gal-1+/+ and Gal-1-/- mice and treated with Gal-1 did not induce lipid bodies. However, the capacity of F1 e -glucan to induce lipid bodies on the peritoneal cells was inhibited by gal-1 treatment. We suggest that the Gal-1 could participate of the development of a protective immune response to H. capsulatum.
ASSUNTO(S)
inflamação inflammation eicosanóides histoplasma capsulatum citocinas galectina-1 eicosanoids modulação da resposta imunológica cytokines histoplasma capsulatum immunological response galectin-1
Documentos Relacionados
- Impact of galectin-1 on the evolution of acute experimental Trypanosoma cruzi infection
- Engagement of galectin-1 in mild hyperhomocysteinemia-induced vascular injury in mice.
- Avaliação da interação entre galectina-1 e zinco e suas potenciais implicações estruturais e funcionais
- Expressão de galectina-1 e -3 na leucemia mielóide crônica e sua contribuição para a progressão da doença.
- Association of galectin-1 and galectin-3 with Gemin4 in complexes containing the SMN protein