Partição da lectina de Canavalia grandiflora Benth em sistemas de duas fases aquosas e aplicação em processo cicatricial / Partition of the lectin from Canavalia grandiflora Benth in aqueous two-phase systems and application healing

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

07/10/2010

RESUMO

The purification process is a limiting step of biomolecules for use in processes in biotechnology, especially lectins, due to the cost of up to 90%, and the large number of steps to obtain the pure molecule, resulting in a low yield. Thus, the aqueous two-phase system (ATPS) was proposed as an alternative for the purification of lectin from Canavalia grandiflora seed (ConGF), because it allowed the rapid separation without denaturation, with low cost and good selectivity. With the aim of understanding the behavior of the partition of the lectin ConGF used a factorial design (24) to partition and recovery using systems formed by polyethylene glycol (PEG) and phosphate and citrate salts, which were evaluated four variables (concentration and PEG molar mass, salt concentration and pH). In addition, tests were performed antimicrobial activity and wound healing potential of the lectin against experimental lesions during 12 days of treatment with a lectin ConGF solution (100μg/mL). The results can be seen that the lectin from Canavalia grandiflora preferentially partitioned to the polymer (PEG) rich phase and the system formed by PEG 400 (g/mol) 20% (w/w), citrate (20% w/w), pH 6.0, the best condition was obtained for the partition coefficient (8.67) and activity yield (104%). For the PEG/ phosphate system, the lectin preferentially partitioned to the salt-rich phase, showing a partition coefficient of 0.03 and activity yield of 1600%. The ATPS proved to be able to recover the ConGF lectin without loss of its activity, demonstrating that a selective system for this protein, allowing this system could be used as the first step for the purification of lectin obtained from the crude extract of the Canavalia grandiflora seeds. The antimicrobial activity was not detected ConGF and lesions in the treated group presentation was macroscopically and microscopically complete healing while the wounds of the control group needed more time toresolution of the healing process

ASSUNTO(S)

sistema de duas fases aquosas lectina, canavalia grandiflora planejamento fatorial peg/citrato cicatrização reepitelização biologia geral aqueous two phase systems, lectin canavalia grandiflora factorial design peg/citrate peg/phosphate wound healing re-epithelialization

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