Oxidative stress in the latissimus dorsi muscle of diabetic rats
AUTOR(ES)
D'Avila, Katia de Angelis Lobo
DATA DE PUBLICAÇÃO
2010
RESUMO
The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 ± 5 g, N = 11). Shortterm (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZdiabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 ± 0.02 nmol/mg protein and 14706 ± 1581 cps/mg protein) than LD muscle of normal rats (0.23 ± 0.04 nmol/mg protein and 7389 ± 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was reduced (58%) in STZ-diabetic rats and superoxide dismutase activity was similar in LD muscle of both groups. A positive correlation was obtained between catalase activity and the oxidative stress of LD, as evaluated in terms of TBARS (r = 0.78) and by chemiluminescence (r = 0.89). Catalase activity also correlated inversely with glutathione peroxidase activity (r = 0.79). These data suggest that an increased oxidative stress in LD muscle of diabetic rats may be related to skeletal muscle myopathy.
ASSUNTO(S)
fisiologia streptozotocin-diabetes latissimus dorsi muscle oxidative stress catalase glutathione
ACESSO AO ARTIGO
http://hdl.handle.net/10183/21175Documentos Relacionados
- Oxidative stress in the latissimus dorsi muscle of diabetic rats
- The fibre type composition of the rabbit latissimus dorsi muscle.
- Effect of Propafenone on the Contractile Activity of Latissimus Dorsi Muscle Isolated in an Organ Chamber: Experimental Study in Rats
- The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats
- Pulmonary oxidative stress in diabetic rats exposed to hyperoxia