Obtenção de extratos vegetais com propriedades funcionais via tecnologia supercritica : uso de CO2 e CO2 + H2O. / Vegetal extracts with functional properties obtained using supercritical technology : use of CO2 and CO2 + H2O.

AUTOR(ES)

Patricia Franco Leal

DATA DE PUBLICAÇÃO

2005

RESUMO

In this work supercritical fluid extraction was used to obtain extracts from Ocimum basilicum (sweet basil), Achyrocline alata, and Achyrocline satureioides; for sweet basil extracts, water was used as cosolvent at 1, 10, and 20% (v/v). The extract?s global yield isotherms, chemical compositions, and antioxidant activities were determined. The extraction assays were done for pressures of 100 to 300 bar at temperatures of 30 to 50 oC. All the experiments were duplicated. The identification of the compounds present in extracts was made by GC-MS. The antioxidant activity of extracts was determined using the coupled reaction of beta-carotene and linolenic acid. The larger global yield of sweet basil extract was 2% (dry basis - d.b.) at 100 bar, 30 oC and 1% of cosolvent H2O (v/v), 11% (d.b.) at 100 and 150 bar, 30 oC and 10% of H2O (v/v), and 24% (d.b.) at 300 bar, 30 oC and 20% H2O. The larger global yield of A. alata extract was 4% (d.b.) and for A. satureioides extract was 2% (d.b.), both at 200 bar and 30 oC. The main components detected in the sweet basil extracts were eugenol, germacrene-D and epi-alpha-cadinol. And, in the A. alata and A. satureioides extracts were trans-caryophyllene and alpha-humulene. The results were compared with low-pressure solvent extraction with ethanol and hydrodistillation. Sweet basil extracts exhibited antioxidant activity smaller as well as larger (99%) than the control (beta-carotene). The antioxidant activity of A. alata extracts obtained by hydrodistillation and supercritical fluid extraction varied from 28% to 100% respectively; the antioxidant activity of A. satureioides extracts obtained by hydrodistillation and supercritical fluid extraction varied from 12 to 98%.

ASSUNTO(S)

atividade antioxidante antioxidan activity achyrocline alata extração com fluido supercritico achyrocline alata supercritical fluid extraction ocimum basilicum achyrocline satureioides achyrocline satureioides ocimum basilicum

Documentos Relacionados

• Extração supercritica de curcuminoides de Curcuma longa L. usando como solvente mistura de CO2 + etanol e/ou isopropanol : um estudo da cinetica e das propriedades funcionais do extrato
• Extraction of stevia glycosides with CO2 + water, CO2 + ethanol, and CO2 + water + ethanol
• Obtenção de extrato de matriz oleaginosa de origem amazonica via tecnologia supercritica : determinação das isotermas de rendimento global, dos parametros de processo e caracterização quimica dos extratos
• Determinação da solubilidade em sistemas pseudo-ternarios : cravo-da-india (Eugenia caryophyllus) + CO2, gengibre (Zinziber Officinale) + CO2 e erva-doce (Pimpinella anisum) + CO2
• Immunomodulatory effects of supercritical fluid CO2 extracts from freeze-dried powder of Tenebrio molitor larvae (yellow mealworm)