Nuclease p1 immobilized on deae cellulose

AUTOR(ES)

Shi, Lu-E, Yi, Yu, Tang, Zhen-Xing, Xiong, Wen-Yue, Mei, Jiang-Feng, Ying, Guo-Qing

FONTE

Brazilian Journal of Chemical Engineering

DATA DE PUBLICAÇÃO

2010-03

RESUMO

Effects of various factors, such as pH, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. The immobilization conditions were optimized by orthogonal experiments. Characterizations of immobilized nuclease p1 were also evaluated. Through orthogonal optimization, the optimal immobilization conditions were as follows: pH 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. Optimal pH of immobilized enzyme was 5.8. Optimal temperature of immobilized enzyme was 70ºC. Thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on DEAE cellulose. Michaelis constant Km of immobilized enzyme at 69ºC was found to be 27.21 g/l by the Lineweaver-Burk plot.

Documentos Relacionados

• Adsorption of nuclease p1 on chitosan nano-particles
• Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.
• P1 nuclease defines a subpopulation of active SV40 chromatin--a new nuclease hypersensitivity assay.
• Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
• PREPARATION AND CHARACTERIZATION OF IMMOBILIZED SPORES WITH LACCASE ACTIVITY FROM Bacillus pumilus W3 ON DEAE-CELLULOSE AND THEIR APPLICATION IN DYE DECOLORIZATION