Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
AUTOR(ES)
Eilat, D
RESUMO
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343591Documentos Relacionados
- Peptidyl-tRNA hydrolase from Sulfolobus solfataricus
- Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.
- Lincosamide antibiotics stimulate dissociation of peptidyl-tRNA from ribosomes.
- Release of (oligo) peptidyl-tRNA from ribosomes by erythromycin A.
- Special peptidyl-tRNA molecules can promote translational frameshifting without slippage.