Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.
AUTOR(ES)
Iverson, B L
RESUMO
2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predominantly at a single guanine position. The mechanism involves methyl-group transfer from sulfur on the modified deoxyuridine of the extended primer to N-7 of guanine on the template DNA. This raises the possibility for the design and synthesis of a nonenzymatic class of sequence-specific methyltransferases for DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=280485Documentos Relacionados
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