Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein.

AUTOR(ES)

Collick, A

RESUMO

Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes.

Documentos Relacionados

• Nonenzymatic sequence-specific cleavage of single-stranded DNA.
• Evidence that replication of human neurotropic JC virus DNA in glial cells is regulated by the sequence-specific single-stranded DNA-binding protein Pur alpha.
• Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.
• Binding of a sequence-specific single-stranded DNA-binding factor to the simian virus 40 core origin inverted repeat domain is cell cycle regulated.
• Agrobacterium tumefaciens virE operon encodes a single-stranded DNA-binding protein.